Effects of sodium nitroprusside on indirect shoot organogenesis and in vitro root formation of Tagetes erecta: an important medicinal plant

Abstract

Tagetes erecta is known as a medicinal, industrial, and ornamental plant that has been used for various diseases due to its broad range of secondary metabolites. Considering the importance and expanding demand for this plant, low viability and poor germination as well as genetic manipulation, tissue culture could be proposed as a solution. Therefore, the aim of this study was to investigate the effect of sodium nitroprusside (SNP) on in vitro shoot regeneration from callus that derived from hypocotyl segments as well as root formation of this valuable plant. Based on our results, Murashige and Skoog (MS) medium supplemented with containing 1.0 mg/l 6-benzylaminopurine (BAP) plus 0.1 mg/l 3-indolebutyric acid (IBA) along with 30 μM SNP had the highest shoot organogenesis frequency (86.66%) and shoot number (10.33) from callus. Moreover, maximum root formation (100%) and root numbers (14.86) were achieved in MS medium containing 60 μM SNP. It seems that the current protocol can be considered as a rapid and reliable protocol for mass production of Marigold